Desorption and ablation regimes in UV-MALDI: the critical fluence.

ABSTRACT: Although MALDI is a widely used technique, there is so far no theoretical description able to reproduce some critical aspects of the experimental results. For example, there is experimental as well as theoretical controversy regarding the minimum laser fluence, i.e., the so-called fluence threshold (F _T), required to evaporate a sample. Furthermore, although the different processes involved in ion production have been the focus of many investigations, the fact is that the primary process for ion formation in MALDI is not desorption but ablation. In this work, we present a new phenomenological approach for understanding MALDI results based on a simple, but physically intuitive, idea consisting of limiting the laser-matter interaction process to three layers. This de

SUBMITTER: Alonso E 

PROVIDER: S-EPMC9809206 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature