Technical versus biological variability in a synthetic human gut community.

ABSTRACT: Synthetic communities grown in well-controlled conditions are an important tool to decipher the mechanisms driving community dynamics. However, replicate time series of synthetic human gut communities in chemostats are rare, and it is thus still an open question to what extent stochasticity impacts gut community dynamics. Here, we address this question with a synthetic human gut bacterial community using an automated fermentation system that allows for a larger number of biological replicates. We collected six biological replicates for a community initially consisting of five common gut bacterial species that fill different metabolic niches. After an initial 12 hours in batch mode, we switched to chemostat mode and observed the community to stabilize after 2-3 days. Community profiling wit

SUBMITTER: van de Velde C 

PROVIDER: S-EPMC9809966 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature