The mRNA vaccine BNT162b2 demonstrates impaired T_H1 immunogenicity in human elders in vitro and aged mice in vivo.

ABSTRACT: mRNA vaccines have been key to addressing the SARS-CoV-2 pandemic but have impaired immunogenicity and durability in vulnerable older populations. We evaluated the mRNA vaccine BNT162b2 in human in vitro whole blood assays with supernatants from adult (18-50 years) and elder (≥60 years) participants measured by mass spectrometry and proximity extension assay proteomics. BNT162b2 induced increased expression of soluble proteins in adult blood (e.g., C1S, PSMC6, CPN1), but demonstrated reduced proteins in elder blood (e.g., TPM4, APOF, APOC2, CPN1, and PI16), including 30-85% lower induction of T_H1-polarizing cytokines and chemokines (e.g., IFNγ, and CXCL10). Elder T_H1 impairment was validated in mice in vivo and associated with impaired humoral and cellul

SUBMITTER: Brook B 

PROVIDER: S-EPMC9810224 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature