Minoxidil Regulates Aging-Like Phenotypes in Rat Cortical Astrocytes In Vitro.

ABSTRACT: Mainly due to the slanted focus on the mechanism and regulation of neuronal aging, research on astrocyte aging and its modulation during brain aging is scarce. In this study, we established aged astrocyte culture model by long-term culturing. Cellular senescence was confirmed through SA-β-gal staining as well as through the examination of morphological, molecular, and functional markers. RNA sequencing and functional analysis of astrocytes were performed to further investigate the detailed characteristics of the aged astrocyte model. Along with aged phenotypes, decreased astrocytic proliferation, migration, mitochondrial energetic function and support for neuronal survival and differentiation has been observed in aged astrocytes. In addition, increased expression of cytokines and chemokine

SUBMITTER: Bang M 

PROVIDER: S-EPMC9810449 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature