The effect of DNA polymorphisms and natural variation on crossover hotspot activity in Arabidopsis hybrids.

ABSTRACT: In hybrid organisms, genetically divergent homologous chromosomes pair and recombine during meiosis; however, the effect of specific types of polymorphisms on crossover is poorly understood. Here, to analyze this in Arabidopsis, we develop the seed-typing method that enables the massively parallel fine-mapping of crossovers by sequencing. We show that structural variants, observed in one of the generated intervals, do not change crossover frequency unless they are located directly within crossover hotspots. Both natural and Cas9-induced deletions result in lower hotspot activity but are not compensated by increases in immediately adjacent hotspots. To examine the effect of single nucleotide polymorphisms on crossover formation, we analyze hotspot activity in mismatch detection-deficient ms

SUBMITTER: Szymanska-Lejman M 

PROVIDER: S-EPMC9810609 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature