Isolation and transfection of myenteric neurons from mice for patch-clamp applications.

ABSTRACT: The enteric nervous system (ENS) is a complex neuronal network organized in ganglionated plexuses that extend along the entire length of the gastrointestinal tract. Largely independent of the central nervous system, the ENS coordinates motility and peristalsis of the digestive tract, regulates secretion and absorption, and is involved in immunological processes. Electrophysiological methods such as the patch-clamp technique are particularly suitable to study the function of neurons as well as the biophysical parameters of the underlying ion channels under both physiological and pathophysiological conditions. However, application of the patch-clamp method to ENS neurons remained difficult because they are embedded in substantial tissue layers that limit access to and targeted manipulation o

SUBMITTER: Kuehs S 

PROVIDER: S-EPMC9810798 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature