Colorectal Cancer Metastases in the Liver Establish Immunosuppressive Spatial Networking between Tumor-Associated SPP1+ Macrophages and Fibroblasts.

ABSTRACT:

Purpose

The liver is the most frequent metastatic site for colorectal cancer. Its microenvironment is modified to provide a niche that is conducive for colorectal cancer cell growth. This study focused on characterizing the cellular changes in the metastatic colorectal cancer (mCRC) liver tumor microenvironment (TME).

Experimental design

We analyzed a series of microsatellite stable (MSS) mCRCs to the liver, paired normal liver tissue, and peripheral blood mononuclear cells using single-cell RNA sequencing (scRNA-seq). We validated our findings using multiplexed spatial imaging and bulk gene expression with cell deconvolution.

Results

We identified TME-specific SPP1-expressing macrophages with altered metabolism features, foam cell characteristics, and increased activ

SUBMITTER: Sathe A 

PROVIDER: S-EPMC9811165 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature