CRISPR/Cas9-mediated inactivation of the phosphatase activity of soluble epoxide hydrolase prevents obesity and cardiac ischemic injury.

ABSTRACT:

Introduction

Although the physiological role of the C-terminal hydrolase domain of the soluble epoxide hydrolase (sEH-H) is well investigated, the function of its N-terminal phosphatase activity (sEH-P) remains unknown.

Objectives

This study aimed to assess in vivo the physiological role of sEH-P.

Methods

CRISPR/Cas9 was used to generate a novel knock-in (KI) rat line lacking the sEH-P activity.

Results

The sEH-P KI rats has a decreased metabolism of lysophosphatidic acids to monoacyglycerols. KI rats grew almost normally but with less weight and fat mass gain while insulin sensitivity was increased compared to wild-type rats. This lean phenotype was more marked in males than in female KI rats and mainly due to decreased food consumption and enhanced energy exp

SUBMITTER: Leuillier M 

PROVIDER: S-EPMC9811321 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature