Direct cloning of a herpesvirus genome for rapid generation of infectious BAC clones.

ABSTRACT:

Introduction

The herpesviridae are DNA viruses with large and complicated genomes. The herpesvirus bacterial artificial chromosomes (BACs) have been useful for generating recombinant viruses to study the biology and pathogenesis. However, the conventional method using homologous recombination is not only time consuming but also prone to accumulate attenuating mutations during serial passage of the virus in cells. Elimination of the BAC vector from the recombinant viral genome requires additional step for phenotypically consistence with the original strain.

Objectives

To generate a streamlined approach for generating infectious BAC clones of herpesvirus.

Methods

The 142-kb pseudorabies virus genome was directly cloned into a bacterial artificial chromosome (BAC) in Esc

SUBMITTER: Yuan H 

PROVIDER: S-EPMC9811322 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature