Depth-Resolved Localization Microangiography in the NIR-II Window.

ABSTRACT: Detailed characterization of microvascular alterations requires high-resolution 3D imaging methods capable of providing both morphological and functional information. Existing optical microscopy tools are routinely used for microangiography, yet offer suboptimal trade-offs between the achievable field of view and spatial resolution with the intense light scattering in biological tissues further limiting the achievable penetration depth. Herein, a new approach for volumetric deep-tissue microangiography based on stereovision combined with super-resolution localization imaging is introduced that overcomes the spatial resolution limits imposed by light diffusion and optical diffraction in wide-field imaging configurations. The method capitalizes on localization and tracking of flowing fluores

SUBMITTER: Zhou Q 

PROVIDER: S-EPMC9811471 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature