Elucidation of microbial lignin degradation pathways using synthetic isotope-labelled lignin.

ABSTRACT: Pathways by which the biopolymer lignin is broken down by soil microbes could be used to engineer new biocatalytic routes from lignin to renewable chemicals, but are currently not fully understood. In order to probe these pathways, we have prepared synthetic lignins containing ¹³C at the sidechain β-carbon. Feeding of [β-¹³C]-labelled DHP lignin to Rhodococcus jostii RHA1 has led to the incorporation of ¹³C label into metabolites oxalic acid, 4-hydroxyphenylacetic acid, and 4-hydroxy-3-methoxyphenylacetic acid, confirming that they are derived from lignin breakdown. We have identified a glycolate oxidase enzyme in Rhodococcus jostii RHA1 which is able to oxidise glycolaldehyde via glycolic acid to oxalic acid, thereby identifying a path

SUBMITTER: Alruwaili A 

PROVIDER: S-EPMC9811514 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature