A label-free light-scattering method to resolve assembly and disassembly of DNA nanostructures.

ABSTRACT: DNA self-assembly, and in particular DNA origami, has evolved into a reliable workhorse for organizing organic and inorganic materials with nanometer precision and with exactly controlled stoichiometry. To ensure the intended performance of a given DNA structure, it is beneficial to determine its folding temperature, which in turn yields the best possible assembly of all DNA strands. Here, we show that temperature-controlled sample holders and standard fluorescence spectrometers or dynamic light-scattering setups in a static light-scattering configuration allow for monitoring the assembly progress in real time. With this robust label-free technique, we determine the folding and melting temperatures of a set of different DNA origami structures without the need for more tedious protocols. In

SUBMITTER: Ijas H 

PROVIDER: S-EPMC9811603 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature