High-throughput morphometric and transcriptomic profiling uncovers composition of naive and sensory-deprived cortical cholinergic VIP/CHAT neurons.

ABSTRACT: Cortical neuronal networks control cognitive output, but their composition and modulation remain elusive. Here, we studied the morphological and transcriptional diversity of cortical cholinergic VIP/ChAT interneurons (VChIs), a sparse population with a largely unknown function. We focused on VChIs from the whole barrel cortex and developed a high-throughput automated reconstruction framework, termed PopRec, to characterize hundreds of VChIs from each mouse in an unbiased manner, while preserving 3D cortical coordinates in multiple cleared mouse brains, accumulating thousands of cells. We identified two fundamentally distinct morphological types of VChIs, bipolar and multipolar that differ in their cortical distribution and general morphological features. Following mild unilateral whisker d

SUBMITTER: Yayon N 

PROVIDER: S-EPMC9811618 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature