Homologous pairing in short double-stranded DNA-grafted colloidal microspheres.

ABSTRACT: Homologous pairing (HP), i.e., the pairing of similar or identical double-stranded DNA, is an insufficiently understood fundamental biological process. HP is now understood to also occur without protein mediation, but crucial mechanistic details remain poorly established. Unfortunately, systematic studies of sequence dependence are not practical due to the enormous number of nucleotide permutations and multiple possible conformations involved in existing biophysical strategies even when using as few as 150 basepairs. Here, we show that HP can occur in DNA as short as 18 basepairs in a colloidal microparticle-based system. Exemplary systematic studies include resolving opposing reports of the impact of % AT composition, validating the impact of nucleotide order and triplet framework and rev

SUBMITTER: Chauhan N 

PROVIDER: S-EPMC9811663 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature