Cohesin and CTCF complexes mediate contacts in chromatin loops depending on nucleosome positions.

ABSTRACT: The spatial organization of the eukaryotic genome plays an important role in regulating transcriptional activity. In the nucleus, chromatin forms loops that assemble into fundamental units called topologically associating domains that facilitate or inhibit long-range contacts. These loops are formed and held together by the ring-shaped cohesin protein complex, and this can involve binding of CCCTC-binding factor (CTCF). High-resolution conformation capture experiments provide the frequency at which two DNA fragments physically associate in three-dimensional space. However, technical limitations of this approach, such as low throughput, low resolution, or noise in contact maps, make data interpretation and identification of chromatin intraloop contacts, e.g., between distal regulatory eleme

SUBMITTER: Attou A 

PROVIDER: S-EPMC9811664 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature