Treatment of Orthotopic U251 Human Glioblastoma Multiforme Tumors in NRG Mice by Convection-Enhanced Delivery of Gold Nanoparticles Labeled with the β-Particle-Emitting Radionuclide, ¹⁷⁷Lu.

ABSTRACT: In this study, we investigated convection-enhanced delivery (CED) of 23 ± 3 nm gold nanoparticles (AuNPs) labeled with the β-particle-emitting radionuclide ¹⁷⁷Lu (¹⁷⁷Lu-AuNPs) for treatment of orthotopic U251-Luc human glioblastoma multiforme (GBM) tumors in NRG mice. The cytotoxicity in vitro of ¹⁷⁷Lu-AuNPs (0.0-2.0 MBq, 4 × 10¹¹ AuNPs) on U251-Luc cells was also studied by a clonogenic survival assay, and DNA double-strand breaks (DSBs) caused by β-particle emissions of ¹⁷⁷Lu were measured by confocal immunofluorescence microscopy for γH2AX. NRG mice with U251-Luc tumors in the right cerebral hemisphere of the brain were treated by CED of 1.1 ± 0.2 MBq of ¹⁷⁷Lu-AuNPs (4 × 10¹¹ AuNPs). Control mice received unlab

SUBMITTER: Georgiou CJ 

PROVIDER: S-EPMC9812026 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature