Surveying the Vampire Bat (<i>Desmodus rotundus</i>) Serum Proteome: A Resource for Identifying Immunological Proteins and Detecting Pathogens.

ABSTRACT: Bats are increasingly studied as model systems for longevity and as natural hosts for some virulent viruses. Yet the ability to characterize immune mechanisms of viral tolerance and to quantify infection dynamics in wild bats is often limited by small sample volumes and few species-specific reagents. Here, we demonstrate how proteomics can overcome these limitations by using data-independent acquisition-based shotgun proteomics to survey the serum proteome of 17 vampire bats (Desmodus rotundus) from Belize. Using just 2 μL of sample and relatively short separations of undepleted serum digests, we identified 361 proteins across 5 orders of magnitude. Levels of immunological proteins in vampire bat serum were then compared to human plasma via published databases. Of particular interes

SUBMITTER: Neely BA 

PROVIDER: S-EPMC9812275 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature