Human serum N-glycome profiling via the newly developed asparagine immobilized cellulose/polymer nanohybrid.

ABSTRACT: Human serum N-linked glycans expression levels change during the disease progression. The low abundance, structural diversity, and coexisting matrices hinder their detection in mass spectrometry analysis. Considering the hydrophilic nature of N-glycans, cellulose/polymer (1,2-Epoxy-5-hexene) nanohybrid is fabricated with oxirane groups functionalized of asparagine to develop solid phase extraction based hydrophilic interaction liquid chromatography sorbent (cellulose/1,2-Epoxy-5-hexene/asparagine). The morphology, elemental analysis, and surface properties are studied through scanning electron microscopy, energy dispersive X-ray spectroscopy, and Fourier-transform infrared spectroscopy. The large surface area of cellulose/polymer nanohybrid (2.09 × 10²  m² /g) facilit

SUBMITTER: Sajid MS 

PROVIDER: S-EPMC9812403 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature