Endoplasmic reticulum expansion throughout the differentiation of teleost B cells to plasmablasts.

ABSTRACT: The differentiation of B cells into antibody-secreting cells is fundamental for the generation of humoral immunity. In mammals, this process involves a series of metabolic and intracellular changes, not studied to date in teleost fish, where a clear distinction between naive B cells and plasmablasts/plasma cells (PCs) is still missing. Thus, in the current study, we have established that upon activation, teleost B cells undergo an expansion of the endoplasmic reticulum (ER) but experience no significant changes in mitochondria content. In parallel, the transcription of genes implicated in B cell differentiation increases, while that of mitochondrial genes decreases. In this context, ER monitoring has allowed us to distinguish between small cells with low amounts of ER (FSC^loER

SUBMITTER: Morel E 

PROVIDER: S-EPMC9812713 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature