Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration.

ABSTRACT: Tissue regeneration requires coordination between resident stem cells and local niche cells^1,2. Here we identify that senescent cells are integral components of the skeletal muscle regenerative niche that repress regeneration at all stages of life. The technical limitation of senescent-cell scarcity³ was overcome by combining single-cell transcriptomics and a senescent-cell enrichment sorting protocol. We identified and isolated different senescent cell types from damaged muscles of young and old mice. Deeper transcriptome, chromatin and pathway analyses revealed conservation of cell identity traits as well as two universal senescence hallmarks (inflammation and fibrosis) across cell type, regeneration time and ageing. Senescent cells create an aged-like inflamed nich

SUBMITTER: Moiseeva V 

PROVIDER: S-EPMC9812788 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature