Methods to Study the Myenteric Plexus of Rat Small Intestine.

ABSTRACT: The close interaction between the enteric nervous system, microbiome, and brain in vertebrates is an emerging topic of recent studies. Different species such as rat, mouse, and human are currently being used for this purpose, among others. The transferability of protocols for tissue isolation and sample collection is not always straightforward. Thus, the present work presents a new protocol for isolation and sample collection of rat myenteric plexus cells for in vivo as well as in vitro studies. With the methods and chemicals described in detail, a wide variety of investigations can be performed with regard to normal physiological as well as pathological processes in the postnatal developing enteric nervous system. The fast and efficient preparation of the intestine as the first step is pa

SUBMITTER: Hecking I 

PROVIDER: S-EPMC9813035 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature