Thermal fluctuations of the lipid membrane determine particle uptake into Giant Unilamellar Vesicles.

ABSTRACT: Phagocytic particle uptake is crucial for the fate of both living cells and pathogens. Invading particles have to overcome fluctuating lipid membranes as the first physical barrier. However, the energy and the role of the fluctuation-based particle-membrane interactions during particle uptake are not understood. We tackle this problem by indenting the membrane of differently composed Giant Unilamellar Vesicles (GUVs) with optically trapped particles until particle uptake. By continuous 1 MHz tracking and autocorrelating the particle's positions within 30µs delays for different indentations, the fluctuations' amplitude, the damping, the mean forces, and the energy profiles were obtained. Remarkably, the uptake energy into a GUV becomes predictable since it increases for smaller fluctuation

SUBMITTER: Ayala YA 

PROVIDER: S-EPMC9813155 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature