Dataset Information

Characteristics of intestinal microbiota in C57BL/6 mice with non-alcoholic fatty liver induced by high-fat diet.

ABSTRACT:

Introduction

As a representation of the gut microbiota, fecal and cecal samples are most often used in human and animal studies, including in non-alcoholic fatty liver disease (NAFLD) research. However, due to the regional structure and function of intestinal microbiota, whether it is representative to use cecal or fecal contents to study intestinal microbiota in the study of NAFLD remains to be shown.

Methods

The NAFLD mouse model was established by high-fat diet induction, and the contents of the jejunum, ileum, cecum, and colon (formed fecal balls) were collected for 16S rRNA gene analysis.

Results

Compared with normal mice, the diversity and the relative abundance of major bacteria and functional genes of the ileum, cecum and colon were significantly changed, but not in the jejunum. In NAFLD mice, the variation characteristics of microbiota in the cecum and colon (feces) were similar. However, the variation characteristics of intestinal microbiota in the ileum and large intestine segments (cecum and colon) were quite different.

Discussion

Therefore, the study results of cecal and colonic (fecal) microbiota cannot completely represent the results of jejunal and ileal microbiota.

SUBMITTER: Yan G

PROVIDER: S-EPMC9813237 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Publications

Characteristics of intestinal microbiota in C57BL/6 mice with non-alcoholic fatty liver induced by high-fat diet.

Yan Guangwen G Li Shuaibing S Wen Yuhang Y Luo Yadan Y Huang Jingrong J Chen Baoting B Lv Shuya S Chen Lang L He Lvqin L He Manli M Yang Qian Q Yu Zehui Z Xiao Wudian W Tang Yong Y Li Weiyao W Han Jianhong J Zhao Fangfang F Yu Shumin S Kong Fang F Abbasi Benazir B Yin Hongmei H Gu Congwei C

Frontiers in microbiology 20221222

<h4>Introduction</h4>As a representation of the gut microbiota, fecal and cecal samples are most often used in human and animal studies, including in non-alcoholic fatty liver disease (NAFLD) research. However, due to the regional structure and function of intestinal microbiota, whether it is representative to use cecal or fecal contents to study intestinal microbiota in the study of NAFLD remains to be shown.<h4>Methods</h4>The NAFLD mouse model was established by high-fat diet induction, and t ...[more]

PMID: 36620001

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Dataset Information

Characteristics of intestinal microbiota in C57BL/6 mice with non-alcoholic fatty liver induced by high-fat diet.

Introduction

Methods

Results

Discussion

Publications

Characteristics of intestinal microbiota in C57BL/6 mice with non-alcoholic fatty liver induced by high-fat diet.

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