Interaction study of aflatoxin M1 with milk proteins using ATR-FTIR.

ABSTRACT: Aflatoxin M₁ (AFM₁) is a metabolite of carcinogenic aflatoxin B₁ (AFB₁) and appears in milk of dairy animals on ingestion of feed contaminated with AFB₁. It has been reported to have affinity towards milk proteins, the exact mechanism of which is still unknown. In the present study, ATR-FTIR coupled with chemometrics is utilized to understand AFM₁ interaction with milk proteins. The second order derivative spectra of the spectral window 1700-1600 cm^-1 confirms the affinity of AFM₁ towards milk proteins. The results of principal component analysis suggested that spectral window of 1700-1600 cm^-1 is informative and provides an indication of the conformational changes brought by AFM₁

SUBMITTER: Harshitha CG 

PROVIDER: S-EPMC9813309 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature