Comparison of DNA extraction methods on CITES-listed timber species and application in species authentication of commercial products using DNA barcoding.

ABSTRACT: Quality and quantity of DNA extracted from wood is important for molecular identification of wood species, which can serve for conservation of wood species and law enforcement to combat illegal wood trading. Rosewood (Dalbergia and Pterocarpus) and agarwood (Aquilaria) are the most commonly found hardwood in timber seizure incidents. To monitor international trade of timber and commercial wood products and to protect these endangered wood species from further population decline, in this study, we have compared three extraction protocols for DNA extraction from 12 samples of rosewood and agarwood timber logs, and later applied the best DNA extraction protocol on 10 commercial wood products claimed to be rosewood and agarwood. We also demonstrated the applicability of DNA mini-barcoding with

SUBMITTER: But GW 

PROVIDER: S-EPMC9813357 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature