The inhibition of PGAM5 suppresses seizures in a kainate-induced epilepsy model via mitophagy reduction.

ABSTRACT:

Background

Epilepsy is a common neurological disease, and excessive mitophagy is considered as one of the major triggers of epilepsy. Mitophagy is a crucial pathway affecting reactive oxygen species. Phosphoglycerate mutase 5 (PGAM5) is a protein phosphatase present in mitochondria that regulates many biological processes including mitophagy and cell death. However, the mechanism of PGAM5 in epilepsy remains unclear. The purpose of the present study was to examine whether PGAM5 affects epilepsy through PTEN-induced putative kinase 1 (PINK1)-mediated mitophagy.

Methods

After the knockdown of PGAM5 expression by the adeno-associated virus, an epilepsy model was created by kainic acid. Next, the seizure activity was recorded by local field potentials before evaluating the level

SUBMITTER: Zhong F 

PROVIDER: S-EPMC9813404 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature