Identification of a novel linear B-cell epitope in porcine deltacoronavirus nucleocapsid protein.

ABSTRACT: Porcine deltacoronavirus (PDCoV) is an emerging swine enteropathogenic coronavirus that caused diarrhea and/or vomiting in neonatal piglets worldwide. Coronaviruses nucleocapsid (N) protein is the most conserved structural protein for viral replication and possesses good antigenicity. In this study, three monoclonal antibodies (mAbs), 3B4, 4D3, and 4E3 identified as subclass IgG2aκ were prepared using the lymphocytic hybridoma technology against PDCoV N protein. Furthermore, the B-cell epitope recognized by mAb 4D3 was mapped by dozens of overlapping truncated recombinant proteins based on the western blotting. The polypeptide ²⁸QFRGNGVPLNSAIKPVE⁴⁴ (EP-4D3) in the N-terminal of PDCoV N protein was identified as the minimal linear epitope for binding mAb 4D3. And the E

SUBMITTER: He W 

PROVIDER: S-EPMC9813470 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature