Creation of human hematopoietic chimeric cell (HHCC) line as a novel strategy for tolerance induction in transplantation.

ABSTRACT:

Background

Cell-based and chimerism-based therapies represent a promising approach for tolerance induction in transplantation. We propose a new cell therapy of the ex vivo created human hematopoietic chimeric cells (HHCC) as an alternative approach to bone marrow (BM)-based therapies in support of solid organ and vascularized composite allotransplantation (VCA). This study aimed to characterize in vitro the phenotype, genotype, clonogenic, and tolerogenic properties of HHCC.

Methods

Thirty ex vivo fusions of CD34⁺ cells from two unrelated human BM donors were performed. CD34⁺ cells were stained separately with PKH26 and PKH67 membrane dyes and fused using polyethylene glycol (PEG). Creation of human HHCC and chimeric state was confi

SUBMITTER: Siemionow M 

PROVIDER: S-EPMC9813662 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature