Humanization of the Shark V_NAR Single Domain Antibody Using CDR Grafting.

ABSTRACT: The variable domain of the new antigen receptor (V_NAR ) of shark single domain antibodies is evolutionarily distant from the variable regions (V_H ) of mammalian immunoglobulins, yet it still has complementarity-determining regions (CDRs) that are involved in antigen recognition, therefore making it possible to humanize by grafting these CDRs to the framework of human V_H homologs. Here, we show the V_NAR CDR based on an analysis of currently available V_NAR -antigen structure complexes in the global Protein Data Bank archive of 3D structure data, and describe the detailed protocol to humanize V_NAR by CDR grafting, using B6 (an anti-Pseudomonas exotoxin V_NAR ), the most common type (Type II) of shark V_NAR s

SUBMITTER: Zhang YF 

PROVIDER: S-EPMC9813873 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature