CUP1 Metallothionein from Healthy Saccharomyces cerevisiae Colocalizes to the Cytosol and Mitochondrial Intermembrane Space.

ABSTRACT: Liquid chromatography, mass spectrometry, and metal analyses of cytosol and mitochondrial filtrates from healthy copper-replete Saccharomyces cerevisiae cells revealed that metallothionein CUP1 was a notable copper-containing species in both compartments, with its abundance dependent upon the level of copper supplementation in the growth media. Electrospray ionization mass spectrometry of cytosol and soluble mitochondrial filtrates displayed a full isotopologue pattern of CUP1 in which the first eight amino acid residues were truncated and eight copper ions were bound. Neither apo-CUP1 nor intermediate copper-bound forms were detected, but chelator treatment could generate apo-CUP1. Mitoplasting revealed that mitochondrial CUP1 was located in the intermembrane space. Fluorescence mi

SUBMITTER: Kim JE 

PROVIDER: S-EPMC9813906 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature