Simple Low-Cost Production of DNA MS2 Virus-Like Particles As Molecular Diagnostic Controls.

ABSTRACT: Suitable controls are integral for the validation and continued quality assurance of diagnostic workflows. Plasmids, DNA, or in vitro transcribed RNA are often used to validate novel diagnostic workflows, however, they are poorly representative of clinical samples. RNA phage virus-like particles (VLPs) packaged with exogenous RNA have been used in clinical diagnostics as workflow controls, serving as surrogates for infectious viral particles. Comparable controls for DNA viruses are more challenging to produce, with analogous DNA phages being infectious and packaging of DNA within RNA phages requiring complex purification procedures and expensive chemical linkers. We present a simple and inexpensive method to produce Emesvirus zinderi (MS2) VLPs, packaged with DNA, that makes

SUBMITTER: Crone MA 

PROVIDER: S-EPMC9814128 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature