The Assembled Genome of the Stroke-Prone Spontaneously Hypertensive Rat.

ABSTRACT:

Background

We report the creation and evaluation of a de novo assembly of the genome of the spontaneously hypertensive rat, the most widely used model of human cardiovascular disease.

Methods

The genome is assembled from long read sequencing (PacBio HiFi and continuous long read data [CLR]) and scaffolded with long-range structural information obtained from Bionano optical maps and proximity ligation sequencing proximity analysis of the genome. The genome assembly was polished with Illumina short reads. Completeness of the assembly was investigated using Benchmarking Universal Single Copy Orthologs analysis. The genome assembly was also evaluated with the rat reference gene set, using NCBI automated protocols. We also generated orthogonal single molecule transcript sequence

SUBMITTER: Kalbfleisch TS 

PROVIDER: S-EPMC9814308 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature