Structural identification of N-glycan isomers using logically derived sequence tandem mass spectrometry.

ABSTRACT: N-linked glycosylation is one of the most important protein post-translational modifications. Despite the importance of N-glycans, the structural determination of N-glycan isomers remains challenging. Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSⁿ), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry. In LODES/MSⁿ, the sequences of successive collision-induced dissociation are derived from carbohydrate dissociation mechanisms and apply to N-glycans in an ion trap for structural determination. We validate LODES/MSⁿ using synthesized N-glycans and subsequently applied this method to N-glycans extracted from soybean, ovalbumin, and IgY. Our me

SUBMITTER: Liew CY 

PROVIDER: S-EPMC9814355 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature