Functionalized Au₁₅ nanoclusters as luminescent probes for protein carbonylation detection.

ABSTRACT: Atomically precise, ligand-protected gold nanoclusters (AuNCs) attract considerable attention as contrast agents in the biosensing field. However, the control of their optical properties and functionalization of surface ligands remain challenging. Here we report a strategy to tailor AuNCs for the precise detection of protein carbonylation-a causal biomarker of ageing. We produce Au₁₅SG₁₃ (SG for glutathione) with atomic precision and functionalize it with a thiolated aminooxy moiety to impart protein carbonyl-binding properties. Mass spectrometry and molecular modelling reveal the key structural features of Au₁₅SG₁₂-Aminooxy and its reactivity towards carbonyls. Finally, we demonstrate that Au₁₅SG₁₂-Aminooxy detects protein

SUBMITTER: Combes GF 

PROVIDER: S-EPMC9814629 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature