A human protein hydroxylase that accepts D-residues.
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ABSTRACT:
SUBMITTER: Choi H
PROVIDER: S-EPMC9814778 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Publications
A human protein hydroxylase that accepts D-residues.
Choi Hwanho H Hardy Adam P AP Leissing Thomas M TM Chowdhury Rasheduzzaman R Nakashima Yu Y Ge Wei W Markoulides Marios M Scotti John S JS Gerken Philip A PA Thorbjornsrud Helen H Kang Dahye D Hong Sungwoo S Lee Joongoo J McDonough Michael A MA Park Hwangseo H Schofield Christopher J CJ
Communications chemistry 20200501 1
Factor inhibiting hypoxia-inducible factor (FIH) is a 2-oxoglutarate-dependent protein hydroxylase that catalyses C3 hydroxylations of protein residues. We report FIH can accept (D)- and (L)-residues for hydroxylation. The substrate selectivity of FIH differs for (D) and (L) epimers, e.g., (D)- but not (L)-allylglycine, and conversely (L)- but not (D)-aspartate, undergo monohydroxylation, in the tested sequence context. The (L)-Leu-containing substrate undergoes FIH-catalysed monohydroxylation, ...[more]
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