Residue-resolved monitoring of protein hyperpolarization at sub-second time resolution

ABSTRACT: Signal-enhancement techniques for NMR spectroscopy are important to amplify the weak resonances provided by nuclear spins. Recently, ‘hyperpolarization’ techniques have been intensively investigated. These provide nuclear spin states far from equilibrium yielding strong signal boosts up to four orders of magnitude. Here we propose a method for real-time NMR of ‘hyperpolarized’ proteins at residue resolution. The approach is based on dissolution dynamic nuclear polarization (d-DNP), which enables the use of hyperpolarized buffers that selectively boost NMR signals of solvent-exposed protein residues. The resulting spectral sparseness and signal enhancements enable recording of residue-resolved spectra at a 2 Hz sampling rate. Thus, we monitor the hyperpolarization level of different protein

SUBMITTER: Negroni M 

PROVIDER: S-EPMC9814832 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature