Rapid detection of cancer DNA in human blood using cysteamine-capped AuNPs and a machine learning-enabled smartphone.

ABSTRACT: DNA methylation occurs when a methyl group is added to a cytosine (C) residue's fifth carbon atom, forming 5-methylcytosine (5-mC). Cancer genomes have a distinct methylation landscape (Methylscape), which could be used as a universal cancer biomarker. This study developed a simple, low-cost, and straightforward Methylscape sensing platform using cysteamine-decorated gold nanoparticles (Cyst/AuNPs), in which the sensing principle is based on methylation-dependent DNA solvation. Normal and cancer DNAs have distinct methylation profiles; thus, they can be distinguished by observing the dispersion of Cyst/AuNPs adsorbed on these DNA aggregates in MgCl₂ solution. After optimising the MgCl₂, Cyst/AuNPs, DNA concentration, and incubation time, the optimised conditions were

SUBMITTER: Koowattanasuchat S 

PROVIDER: S-EPMC9814906 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature