Scaling-down biopharmaceutical production processes via a single multi-compartment bioreactor (SMCB).

ABSTRACT: Biopharmaceutical production processes often use mammalian cells in bioreactors larger than 10,000 L, where gradients of shear stress, substrate, dissolved oxygen and carbon dioxide, and pH are likely to occur. As former tissue cells, producer cell lines such as Chinese hamster ovary (CHO) cells sensitively respond to these mixing heterogeneities, resulting in related scenarios being mimicked in scale-down reactors. However, commonly applied multi-compartment approaches comprising multiple reactors impose a biasing shear stress caused by pumping. The latter can be prevented using the single multi-compartment bioreactor (SMCB) presented here. The exchange area provided by a disc mounted between the upper and lower compartments in a stirred bioreactor was found to be an essential design para

SUBMITTER: Gaugler L 

PROVIDER: S-EPMC9815078 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature