Synthetic mutualism in engineered E. coli mutant strains as functional basis for microbial production consortia.

ABSTRACT: In nature, microorganisms often reside in symbiotic co-existence providing nutrition, stability, and protection for each partner by applying "division of labor." This principle may also be used for the overproduction of targeted compounds in bioprocesses. It requires the engineering of a synthetic co-culture with distributed tasks for each partner. Thereby, the competition on precursors, redox cofactors, and energy-which occurs in a single host-is prevented. Current applications often focus on unidirectional interactions, that is, the product of partner A is used for the completion of biosynthesis by partner B. Here, we present a synthetically engineered Escherichia coli co-culture of two engineered mutant strains marked by the essential interaction of the partners which is achieved

SUBMITTER: Muller T 

PROVIDER: S-EPMC9815082 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature