Assessing the growth kinetics and stoichiometry of Escherichia coli at the single-cell level.

ABSTRACT: Microfluidic cultivation and single-cell analysis are inherent parts of modern microbial biotechnology and microbiology. However, implementing biochemical engineering principles based on the kinetics and stoichiometry of growth in microscopic spaces remained unattained. We here present a novel integrated framework that utilizes distinct microfluidic cultivation technologies and single-cell analytics to make the fundamental math of process-oriented biochemical engineering applicable at the single-cell level. A combination of non-invasive optical cell mass determination with sub-pg sensitivity, microfluidic perfusion cultivations for establishing physiological steady-states, and picoliter batch reactors, enabled the quantification of all physiological parameters relevant to approximate a mat

SUBMITTER: Smaluch K 

PROVIDER: S-EPMC9815083 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature