Novel neutralizing chicken IgY antibodies targeting 17 potent conserved peptides identified by SARS-CoV-2 proteome microarray, and future prospects.

ABSTRACT:

Introduction

An approach toward novel neutralizing IgY polyclonal antibodies (N-IgY-pAb) against SARS-CoV-2 S-ECD was developed.

Material and methods

The novel N-IgY-pAb and its intranasal spray response against the wild type ("'WH-Human 1") SARS-CoV-2 virus, variants of Delta or Omicron were up to 98%. Unique virus peptides binding to N-IgY-pAb were screened by a SARS-CoV-2 proteome microarray.

Results

Seventeen mutation-free peptides with a Z-score > 3.0 were identified as potent targets from a total of 966 peptides. The new findings show that one is in the RBM domain (⁴⁶¹LKPFERDISTEIYQA⁴⁷⁵ ), two are in the NTD domain (²¹RTQLPPAYTNSFTRG³⁵, ²⁹¹CALDPLSETKCTLKS³⁰⁵) four are in the C1/2-terminal (⁵⁶

SUBMITTER: Li J 

PROVIDER: S-EPMC9815496 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature