Myriocin enhances the antifungal activity of fluconazole by blocking the membrane localization of the efflux pump Cdr1.

ABSTRACT: Introduction: Extrusion of azoles from the cell, mediated by an efflux pump Cdr1, is one of the most frequently used strategies for developing azole resistance in pathogenic fungi. The efflux pump Cdr1 is predominantly localized in lipid rafts within the plasma membrane, and its localization is sensitive to changes in the composition of lipid rafts. Our previous study found that the calcineurin signal pathway is important in transferring sphingolipids from the inner to the outer membrane. Methods: We investigated multiple factors that enhance the antifungal activity of fluconazole (FLC) using minimum inhibitory concentration (MIC) assays and disk diffusion assays. We studied the mechanism of action of myriocin through qRT-PCR analysis and confocal microscopy analysis. We test

SUBMITTER: Wang H 

PROVIDER: S-EPMC9815617 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature