Exploring a diverse world of effector domains and amyloid signaling motifs in fungal NLR proteins.

ABSTRACT: NLR proteins are intracellular receptors constituting a conserved component of the innate immune system of cellular organisms. In fungi, NLRs are characterized by high diversity of architectures and presence of amyloid signaling. Here, we explore the diverse world of effector and signaling domains of fungal NLRs using state-of-the-art bioinformatic methods including MMseqs2 for fast clustering, probabilistic context-free grammars for sequence analysis, and AlphaFold2 deep neural networks for structure prediction. In addition to substantially improving the overall annotation, especially in basidiomycetes, the study identifies novel domains and reveals the structural similarity of MLKL-related HeLo- and Goodbye-like domains forming the most abundant superfamily of fungal NLR effectors. Moreo

SUBMITTER: Wojciechowski JW 

PROVIDER: S-EPMC9815663 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature