Pectin-based bioinks for 3D models of neural tissue produced by a pH-controlled kinetics.

ABSTRACT: Introduction: In the view of 3D-bioprinting with cell models representative of neural cells, we produced inks to mimic the basic viscoelastic properties of brain tissue. Moving from the concept that rheology provides useful information to predict ink printability, this study improves and expands the potential of the previously published 3D-reactive printing approach by introducing pH as a key parameter to be controlled, together with printing time. Methods: The viscoelastic properties, printability, and microstructure of pectin gels crosslinked with CaCO₃ were investigated and their composition was optimized (i.e., by including cell culture medium, HEPES buffer, and collagen). Different cell models representative of the major brain cell populations (i.e., neurons,

SUBMITTER: Merli M 

PROVIDER: S-EPMC9815771 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature