Project description:In this study, we have identified candidates for IFN-M-NM-3-inducible CTLs-suppressive factors in melanoma cells using cDNA microarray analysis Double-stranded cDNAs were synthesized from mRNAs extracted from melanoma cell lines that express melanoma-associated antigen recognized by T cells 1 (MART-1) and HLA-A*0201 (526mel, 1833mel, 2487mel, and 501Amel), with or without pretreatment with recombinant human IFN-M-NM-3 (rhIFN-M-NM-3, 1000 U/mL, 48hours).
Project description:In this study, we have identified candidates for IFN-γ-inducible CTLs-suppressive factors in melanoma cells using cDNA microarray analysis