The intestinal permeability marker FITC-dextran 4kDa should be dosed according to lean body mass in obese mice.

ABSTRACT:

Aims

To investigate the influence of the dose in the FITC-Dextran 4kDa (FD-4) permeability test in an obese mouse model, we tested the bodyweight dose regimen and a lean body mass-based dose regimen in high fat diet (HFD) mice and low fat diet (LFD) mice. We hypothesized that the FD-4 permeation result would be dose-dependent.

Methods

The two dose regimens were compared in HFD and LFD mice. Furthermore, we conducted a dose-response study to test the effect of a low or high dose of FD-4 in weight-stratified lean mice. Gene analysis of tight junctions was also carried out.

Results

The FD-4 intestinal permeability test was dose-dependent as we found a significant increase in plasma levels of FD-4 in obese mice with the bodyweight dose regimen. However, this difference wa

SUBMITTER: Voetmann LM 

PROVIDER: S-EPMC9816099 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature