Efficient hyperactive piggyBac transgenesis in Plodia pantry moths.

ABSTRACT: While piggyBac transposon-based transgenesis is widely used in various emerging model organisms, its relatively low transposition rate in butterflies and moths has hindered its use for routine genetic transformation in Lepidoptera. Here, we tested the suitability of a codon-optimized hyperactive piggyBac transposase (hyPBase) in mRNA form to deliver and integrate transgenic cassettes into the genome of the pantry moth Plodia interpunctella. Co-injection of hyPBase mRNA with donor plasmids successfully integrated 1.5-4.4 kb expression cassettes driving the fluorescent markers EGFP, DsRed, or EYFP in eyes and glia with the 3xP3 promoter. Somatic integration and expression of the transgene in the G₀ injected generation was detectable from 7

SUBMITTER: Heryanto C 

PROVIDER: S-EPMC9816379 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature