The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth.

ABSTRACT:

Introduction

To survive in various hostile environments, two-component system is an adaptive mechanism for diverse bacteria. Activity of the CpxA/CpxR two-component system contributes to coping with different stimuli, such as pH, osmotic and heat stress.

Methods

However, the role of the CpxA/CpxR system in cold resistance is little-known. In this study, we showed that CpxA/CpxRwas critical for A. pleuropneumoniae growth under cold stress.

Results

β-Galactosidaseanalysis showed that CpxA/CpxR positively regulated the predicted cold stress gene cspC. The mutant for cold stress gene cspC was impaired in the optimal growth of A. pleuropneumoniae under cold stress. Furthermore, electrophoretic mobility shift assays demonstrated that CpxR-

SUBMITTER: Yao Q 

PROVIDER: S-EPMC9816388 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature