A novel DNA detection using spherical identification probe and strand displacement reaction-initiated silver nanocluster switch.

ABSTRACT: Herein, we developed a novel fluorescent assay using spherical identification probes and toehold-mediated strand displacement reaction-initiated silver nanoclusters (AgNCs) "on-off" signal switch. In this strategy, the target was captured by the spherical probes to induce the activity of exonuclease III (Exo III), catalyzing the cyclic cleavage of substrates to produce a mass of trigger strands. After magnetic bead separation, the intermediates in the supernatant activated downstream toehold-mediated strand displacement reaction to change the structure of silver nanocluster templates, leading to fluorescence intensity reduction. Furthermore, it is demonstrated that the application of spherical identification probes could reduce the signal leakage and the limit of detection. In addition, Ag

SUBMITTER: Yang X 

PROVIDER: S-EPMC9816546 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature