Pilot testing and optimization of a larval fathead minnow high throughput transcriptomics assay.

ABSTRACT: Concentrations at which global gene expression profiles in cells or animals exposed to a test substance start to differ significantly from those of controls have been proposed as an alternative point of departure for use in screening level hazard assessment. The present study describes pilot testing of a high throughput compatible transcriptomics assay with larval fathead minnows. One day post hatch fathead minnows were exposed to eleven different concentrations of three metals, three selective serotonin reuptake inhibitors, and four neonicotinoid-like compounds for 24 h and concentration response modeling was applied to whole body gene expression data. Transcriptomics-based points of departure (tPODs) were consistently lower than effect concentrations reported in apical endpoint studies i

SUBMITTER: Villeneuve DL 

PROVIDER: S-EPMC9816907 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature